hg dmem Search Results


hg-dmem  (EuroClone)
90
EuroClone hg-dmem
Hg Dmem, supplied by EuroClone, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
hg-dmem - by Bioz Stars, 2026-03
90/100 stars
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dmem-hg  (Welgene inc)
90
Welgene inc dmem-hg
Dmem Hg, supplied by Welgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dmem-hg - by Bioz Stars, 2026-03
90/100 stars
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dmem-hg  (FUJIFILM)
90
FUJIFILM dmem-hg
Dmem Hg, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dmem-hg - by Bioz Stars, 2026-03
90/100 stars
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dmem-hg medium  (Biosera Ltd)
90
Biosera Ltd dmem-hg medium
Dmem Hg Medium, supplied by Biosera Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
dmem-hg medium - by Bioz Stars, 2026-03
90/100 stars
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dmem-hg  (Lonza)
90
Lonza dmem-hg
Dmem Hg, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dmem-hg/product/Lonza
Average 90 stars, based on 1 article reviews
dmem-hg - by Bioz Stars, 2026-03
90/100 stars
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dmem hg medium  (Merck & Co)
90
Merck & Co dmem hg medium
The cells were exposed to the extracts obtained by incubating alginate formulations in a <t>DMEM</t> <t>HG</t> medium. Each test was performed in 4 replicates; error bars represent the standard deviation (SD). Statistical analysis was carried out using one-way ANOVA followed by post-hoc Tukey tests; the significant differences relative to the CONTROL are marked with an asterisk *. The treatments are denoted as follows: CONTRO L - cells cultured in DMEM HG without serum; FBS - cells cultured in DMEM HG with 10% fetal bovine serum; TRITON - cells cultured in DMEM HG with 1% (v/v) Triton X100; ALG200 - 200 µl of extract from pure 2% sodium alginate formulation ALG100 - 100 µl of extract from 2% sodium alginate + 100 µl of DMEM HG; ZEB200 - 200 µl of extract from 2% sodium alginate containing zebularine (240 mg per 1 ml); ZEB100 - 100 µl of extract from 2% sodium alginate containing zebularine (240 mg per 1 ml) + 100 µl of DMEM HG; ZEB100+ALG100 - 100 µl of extract from 2% sodium alginate containing zebularine (240 mg per 1 ml) + 100 µl of extract from 2% sodium alginate; ZEB50+ALG50 - 50 µl of extract from 2% sodium alginate containing zebularine (240 mg per 1 ml) + 50 µl of extract from 2% sodium alginate + 100 µl of DMEM HG; RA200 - 200 µl of extract from 2% sodium alginate containing retinoic acid (4.0 mg per 1 ml); RA100 - 100 µl of extract from 2% sodium alginate containing retinoic acid (4.0 mg per 1 ml) + 100 µl of DMEM HG; RA100+ALG100 - 100 µl of extract from 2% sodium alginate containing retinoic acid (4.0 mg per 1 ml) + 100 µl of extract from 2% sodium alginate; RA50+ALG50 - 50 µl of extract from 2% sodium alginate containing retinoic acid (4.0 mg per 1 ml) + 50 µl of extract from 2% sodium alginate + 100 µl of DMEM HG; ZEB100+RA100 - 100 µl of extract from 2% sodium alginate containing zebularine (240 mg per 1 ml) + 100 µl of extract from 2% sodium alginate containing retinoic acid (4.0 mg per 1 ml); ZEB50+RA50 - 50 µl of extract from 2% sodium alginate containing zebularine (240 mg per 1 ml) + 50 µl of extract from 2% sodium alginate containing retinoic acid (4.0 mg per 1 ml) + 100 µl of DMEM HG.
Dmem Hg Medium, supplied by Merck & Co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dmem hg medium/product/Merck & Co
Average 90 stars, based on 1 article reviews
dmem hg medium - by Bioz Stars, 2026-03
90/100 stars
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hg-dmem 11965092  (Fisher Scientific)
90
Fisher Scientific hg-dmem 11965092
The cells were exposed to the extracts obtained by incubating alginate formulations in a <t>DMEM</t> <t>HG</t> medium. Each test was performed in 4 replicates; error bars represent the standard deviation (SD). Statistical analysis was carried out using one-way ANOVA followed by post-hoc Tukey tests; the significant differences relative to the CONTROL are marked with an asterisk *. The treatments are denoted as follows: CONTRO L - cells cultured in DMEM HG without serum; FBS - cells cultured in DMEM HG with 10% fetal bovine serum; TRITON - cells cultured in DMEM HG with 1% (v/v) Triton X100; ALG200 - 200 µl of extract from pure 2% sodium alginate formulation ALG100 - 100 µl of extract from 2% sodium alginate + 100 µl of DMEM HG; ZEB200 - 200 µl of extract from 2% sodium alginate containing zebularine (240 mg per 1 ml); ZEB100 - 100 µl of extract from 2% sodium alginate containing zebularine (240 mg per 1 ml) + 100 µl of DMEM HG; ZEB100+ALG100 - 100 µl of extract from 2% sodium alginate containing zebularine (240 mg per 1 ml) + 100 µl of extract from 2% sodium alginate; ZEB50+ALG50 - 50 µl of extract from 2% sodium alginate containing zebularine (240 mg per 1 ml) + 50 µl of extract from 2% sodium alginate + 100 µl of DMEM HG; RA200 - 200 µl of extract from 2% sodium alginate containing retinoic acid (4.0 mg per 1 ml); RA100 - 100 µl of extract from 2% sodium alginate containing retinoic acid (4.0 mg per 1 ml) + 100 µl of DMEM HG; RA100+ALG100 - 100 µl of extract from 2% sodium alginate containing retinoic acid (4.0 mg per 1 ml) + 100 µl of extract from 2% sodium alginate; RA50+ALG50 - 50 µl of extract from 2% sodium alginate containing retinoic acid (4.0 mg per 1 ml) + 50 µl of extract from 2% sodium alginate + 100 µl of DMEM HG; ZEB100+RA100 - 100 µl of extract from 2% sodium alginate containing zebularine (240 mg per 1 ml) + 100 µl of extract from 2% sodium alginate containing retinoic acid (4.0 mg per 1 ml); ZEB50+RA50 - 50 µl of extract from 2% sodium alginate containing zebularine (240 mg per 1 ml) + 50 µl of extract from 2% sodium alginate containing retinoic acid (4.0 mg per 1 ml) + 100 µl of DMEM HG.
Hg Dmem 11965092, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hg-dmem 11965092/product/Fisher Scientific
Average 90 stars, based on 1 article reviews
hg-dmem 11965092 - by Bioz Stars, 2026-03
90/100 stars
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dmem-hg  (Biochrom)
90
Biochrom dmem-hg
The cells were exposed to the extracts obtained by incubating alginate formulations in a <t>DMEM</t> <t>HG</t> medium. Each test was performed in 4 replicates; error bars represent the standard deviation (SD). Statistical analysis was carried out using one-way ANOVA followed by post-hoc Tukey tests; the significant differences relative to the CONTROL are marked with an asterisk *. The treatments are denoted as follows: CONTRO L - cells cultured in DMEM HG without serum; FBS - cells cultured in DMEM HG with 10% fetal bovine serum; TRITON - cells cultured in DMEM HG with 1% (v/v) Triton X100; ALG200 - 200 µl of extract from pure 2% sodium alginate formulation ALG100 - 100 µl of extract from 2% sodium alginate + 100 µl of DMEM HG; ZEB200 - 200 µl of extract from 2% sodium alginate containing zebularine (240 mg per 1 ml); ZEB100 - 100 µl of extract from 2% sodium alginate containing zebularine (240 mg per 1 ml) + 100 µl of DMEM HG; ZEB100+ALG100 - 100 µl of extract from 2% sodium alginate containing zebularine (240 mg per 1 ml) + 100 µl of extract from 2% sodium alginate; ZEB50+ALG50 - 50 µl of extract from 2% sodium alginate containing zebularine (240 mg per 1 ml) + 50 µl of extract from 2% sodium alginate + 100 µl of DMEM HG; RA200 - 200 µl of extract from 2% sodium alginate containing retinoic acid (4.0 mg per 1 ml); RA100 - 100 µl of extract from 2% sodium alginate containing retinoic acid (4.0 mg per 1 ml) + 100 µl of DMEM HG; RA100+ALG100 - 100 µl of extract from 2% sodium alginate containing retinoic acid (4.0 mg per 1 ml) + 100 µl of extract from 2% sodium alginate; RA50+ALG50 - 50 µl of extract from 2% sodium alginate containing retinoic acid (4.0 mg per 1 ml) + 50 µl of extract from 2% sodium alginate + 100 µl of DMEM HG; ZEB100+RA100 - 100 µl of extract from 2% sodium alginate containing zebularine (240 mg per 1 ml) + 100 µl of extract from 2% sodium alginate containing retinoic acid (4.0 mg per 1 ml); ZEB50+RA50 - 50 µl of extract from 2% sodium alginate containing zebularine (240 mg per 1 ml) + 50 µl of extract from 2% sodium alginate containing retinoic acid (4.0 mg per 1 ml) + 100 µl of DMEM HG.
Dmem Hg, supplied by Biochrom, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dmem-hg/product/Biochrom
Average 90 stars, based on 1 article reviews
dmem-hg - by Bioz Stars, 2026-03
90/100 stars
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dmem (hg)  (Corning Life Sciences)
90
Corning Life Sciences dmem (hg)
The cells were exposed to the extracts obtained by incubating alginate formulations in a <t>DMEM</t> <t>HG</t> medium. Each test was performed in 4 replicates; error bars represent the standard deviation (SD). Statistical analysis was carried out using one-way ANOVA followed by post-hoc Tukey tests; the significant differences relative to the CONTROL are marked with an asterisk *. The treatments are denoted as follows: CONTRO L - cells cultured in DMEM HG without serum; FBS - cells cultured in DMEM HG with 10% fetal bovine serum; TRITON - cells cultured in DMEM HG with 1% (v/v) Triton X100; ALG200 - 200 µl of extract from pure 2% sodium alginate formulation ALG100 - 100 µl of extract from 2% sodium alginate + 100 µl of DMEM HG; ZEB200 - 200 µl of extract from 2% sodium alginate containing zebularine (240 mg per 1 ml); ZEB100 - 100 µl of extract from 2% sodium alginate containing zebularine (240 mg per 1 ml) + 100 µl of DMEM HG; ZEB100+ALG100 - 100 µl of extract from 2% sodium alginate containing zebularine (240 mg per 1 ml) + 100 µl of extract from 2% sodium alginate; ZEB50+ALG50 - 50 µl of extract from 2% sodium alginate containing zebularine (240 mg per 1 ml) + 50 µl of extract from 2% sodium alginate + 100 µl of DMEM HG; RA200 - 200 µl of extract from 2% sodium alginate containing retinoic acid (4.0 mg per 1 ml); RA100 - 100 µl of extract from 2% sodium alginate containing retinoic acid (4.0 mg per 1 ml) + 100 µl of DMEM HG; RA100+ALG100 - 100 µl of extract from 2% sodium alginate containing retinoic acid (4.0 mg per 1 ml) + 100 µl of extract from 2% sodium alginate; RA50+ALG50 - 50 µl of extract from 2% sodium alginate containing retinoic acid (4.0 mg per 1 ml) + 50 µl of extract from 2% sodium alginate + 100 µl of DMEM HG; ZEB100+RA100 - 100 µl of extract from 2% sodium alginate containing zebularine (240 mg per 1 ml) + 100 µl of extract from 2% sodium alginate containing retinoic acid (4.0 mg per 1 ml); ZEB50+RA50 - 50 µl of extract from 2% sodium alginate containing zebularine (240 mg per 1 ml) + 50 µl of extract from 2% sodium alginate containing retinoic acid (4.0 mg per 1 ml) + 100 µl of DMEM HG.
Dmem (Hg), supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dmem (hg)/product/Corning Life Sciences
Average 90 stars, based on 1 article reviews
dmem (hg) - by Bioz Stars, 2026-03
90/100 stars
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dmem-hg media  (HiMedia Laboratories)
90
HiMedia Laboratories dmem-hg media
The cells were exposed to the extracts obtained by incubating alginate formulations in a <t>DMEM</t> <t>HG</t> medium. Each test was performed in 4 replicates; error bars represent the standard deviation (SD). Statistical analysis was carried out using one-way ANOVA followed by post-hoc Tukey tests; the significant differences relative to the CONTROL are marked with an asterisk *. The treatments are denoted as follows: CONTRO L - cells cultured in DMEM HG without serum; FBS - cells cultured in DMEM HG with 10% fetal bovine serum; TRITON - cells cultured in DMEM HG with 1% (v/v) Triton X100; ALG200 - 200 µl of extract from pure 2% sodium alginate formulation ALG100 - 100 µl of extract from 2% sodium alginate + 100 µl of DMEM HG; ZEB200 - 200 µl of extract from 2% sodium alginate containing zebularine (240 mg per 1 ml); ZEB100 - 100 µl of extract from 2% sodium alginate containing zebularine (240 mg per 1 ml) + 100 µl of DMEM HG; ZEB100+ALG100 - 100 µl of extract from 2% sodium alginate containing zebularine (240 mg per 1 ml) + 100 µl of extract from 2% sodium alginate; ZEB50+ALG50 - 50 µl of extract from 2% sodium alginate containing zebularine (240 mg per 1 ml) + 50 µl of extract from 2% sodium alginate + 100 µl of DMEM HG; RA200 - 200 µl of extract from 2% sodium alginate containing retinoic acid (4.0 mg per 1 ml); RA100 - 100 µl of extract from 2% sodium alginate containing retinoic acid (4.0 mg per 1 ml) + 100 µl of DMEM HG; RA100+ALG100 - 100 µl of extract from 2% sodium alginate containing retinoic acid (4.0 mg per 1 ml) + 100 µl of extract from 2% sodium alginate; RA50+ALG50 - 50 µl of extract from 2% sodium alginate containing retinoic acid (4.0 mg per 1 ml) + 50 µl of extract from 2% sodium alginate + 100 µl of DMEM HG; ZEB100+RA100 - 100 µl of extract from 2% sodium alginate containing zebularine (240 mg per 1 ml) + 100 µl of extract from 2% sodium alginate containing retinoic acid (4.0 mg per 1 ml); ZEB50+RA50 - 50 µl of extract from 2% sodium alginate containing zebularine (240 mg per 1 ml) + 50 µl of extract from 2% sodium alginate containing retinoic acid (4.0 mg per 1 ml) + 100 µl of DMEM HG.
Dmem Hg Media, supplied by HiMedia Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dmem-hg media/product/HiMedia Laboratories
Average 90 stars, based on 1 article reviews
dmem-hg media - by Bioz Stars, 2026-03
90/100 stars
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dmem-hg medium  (Cambrex)
90
Cambrex dmem-hg medium
The cells were exposed to the extracts obtained by incubating alginate formulations in a <t>DMEM</t> <t>HG</t> medium. Each test was performed in 4 replicates; error bars represent the standard deviation (SD). Statistical analysis was carried out using one-way ANOVA followed by post-hoc Tukey tests; the significant differences relative to the CONTROL are marked with an asterisk *. The treatments are denoted as follows: CONTRO L - cells cultured in DMEM HG without serum; FBS - cells cultured in DMEM HG with 10% fetal bovine serum; TRITON - cells cultured in DMEM HG with 1% (v/v) Triton X100; ALG200 - 200 µl of extract from pure 2% sodium alginate formulation ALG100 - 100 µl of extract from 2% sodium alginate + 100 µl of DMEM HG; ZEB200 - 200 µl of extract from 2% sodium alginate containing zebularine (240 mg per 1 ml); ZEB100 - 100 µl of extract from 2% sodium alginate containing zebularine (240 mg per 1 ml) + 100 µl of DMEM HG; ZEB100+ALG100 - 100 µl of extract from 2% sodium alginate containing zebularine (240 mg per 1 ml) + 100 µl of extract from 2% sodium alginate; ZEB50+ALG50 - 50 µl of extract from 2% sodium alginate containing zebularine (240 mg per 1 ml) + 50 µl of extract from 2% sodium alginate + 100 µl of DMEM HG; RA200 - 200 µl of extract from 2% sodium alginate containing retinoic acid (4.0 mg per 1 ml); RA100 - 100 µl of extract from 2% sodium alginate containing retinoic acid (4.0 mg per 1 ml) + 100 µl of DMEM HG; RA100+ALG100 - 100 µl of extract from 2% sodium alginate containing retinoic acid (4.0 mg per 1 ml) + 100 µl of extract from 2% sodium alginate; RA50+ALG50 - 50 µl of extract from 2% sodium alginate containing retinoic acid (4.0 mg per 1 ml) + 50 µl of extract from 2% sodium alginate + 100 µl of DMEM HG; ZEB100+RA100 - 100 µl of extract from 2% sodium alginate containing zebularine (240 mg per 1 ml) + 100 µl of extract from 2% sodium alginate containing retinoic acid (4.0 mg per 1 ml); ZEB50+RA50 - 50 µl of extract from 2% sodium alginate containing zebularine (240 mg per 1 ml) + 50 µl of extract from 2% sodium alginate containing retinoic acid (4.0 mg per 1 ml) + 100 µl of DMEM HG.
Dmem Hg Medium, supplied by Cambrex, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
dmem-hg medium - by Bioz Stars, 2026-03
90/100 stars
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dmem-hg/1.5 g/l sodium bicarbonate medium  (Colorado Serum Company)
90
Colorado Serum Company dmem-hg/1.5 g/l sodium bicarbonate medium
The cells were exposed to the extracts obtained by incubating alginate formulations in a <t>DMEM</t> <t>HG</t> medium. Each test was performed in 4 replicates; error bars represent the standard deviation (SD). Statistical analysis was carried out using one-way ANOVA followed by post-hoc Tukey tests; the significant differences relative to the CONTROL are marked with an asterisk *. The treatments are denoted as follows: CONTRO L - cells cultured in DMEM HG without serum; FBS - cells cultured in DMEM HG with 10% fetal bovine serum; TRITON - cells cultured in DMEM HG with 1% (v/v) Triton X100; ALG200 - 200 µl of extract from pure 2% sodium alginate formulation ALG100 - 100 µl of extract from 2% sodium alginate + 100 µl of DMEM HG; ZEB200 - 200 µl of extract from 2% sodium alginate containing zebularine (240 mg per 1 ml); ZEB100 - 100 µl of extract from 2% sodium alginate containing zebularine (240 mg per 1 ml) + 100 µl of DMEM HG; ZEB100+ALG100 - 100 µl of extract from 2% sodium alginate containing zebularine (240 mg per 1 ml) + 100 µl of extract from 2% sodium alginate; ZEB50+ALG50 - 50 µl of extract from 2% sodium alginate containing zebularine (240 mg per 1 ml) + 50 µl of extract from 2% sodium alginate + 100 µl of DMEM HG; RA200 - 200 µl of extract from 2% sodium alginate containing retinoic acid (4.0 mg per 1 ml); RA100 - 100 µl of extract from 2% sodium alginate containing retinoic acid (4.0 mg per 1 ml) + 100 µl of DMEM HG; RA100+ALG100 - 100 µl of extract from 2% sodium alginate containing retinoic acid (4.0 mg per 1 ml) + 100 µl of extract from 2% sodium alginate; RA50+ALG50 - 50 µl of extract from 2% sodium alginate containing retinoic acid (4.0 mg per 1 ml) + 50 µl of extract from 2% sodium alginate + 100 µl of DMEM HG; ZEB100+RA100 - 100 µl of extract from 2% sodium alginate containing zebularine (240 mg per 1 ml) + 100 µl of extract from 2% sodium alginate containing retinoic acid (4.0 mg per 1 ml); ZEB50+RA50 - 50 µl of extract from 2% sodium alginate containing zebularine (240 mg per 1 ml) + 50 µl of extract from 2% sodium alginate containing retinoic acid (4.0 mg per 1 ml) + 100 µl of DMEM HG.
Dmem Hg/1.5 G/L Sodium Bicarbonate Medium, supplied by Colorado Serum Company, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dmem-hg/1.5 g/l sodium bicarbonate medium/product/Colorado Serum Company
Average 90 stars, based on 1 article reviews
dmem-hg/1.5 g/l sodium bicarbonate medium - by Bioz Stars, 2026-03
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Image Search Results


The cells were exposed to the extracts obtained by incubating alginate formulations in a DMEM HG medium. Each test was performed in 4 replicates; error bars represent the standard deviation (SD). Statistical analysis was carried out using one-way ANOVA followed by post-hoc Tukey tests; the significant differences relative to the CONTROL are marked with an asterisk *. The treatments are denoted as follows: CONTRO L - cells cultured in DMEM HG without serum; FBS - cells cultured in DMEM HG with 10% fetal bovine serum; TRITON - cells cultured in DMEM HG with 1% (v/v) Triton X100; ALG200 - 200 µl of extract from pure 2% sodium alginate formulation ALG100 - 100 µl of extract from 2% sodium alginate + 100 µl of DMEM HG; ZEB200 - 200 µl of extract from 2% sodium alginate containing zebularine (240 mg per 1 ml); ZEB100 - 100 µl of extract from 2% sodium alginate containing zebularine (240 mg per 1 ml) + 100 µl of DMEM HG; ZEB100+ALG100 - 100 µl of extract from 2% sodium alginate containing zebularine (240 mg per 1 ml) + 100 µl of extract from 2% sodium alginate; ZEB50+ALG50 - 50 µl of extract from 2% sodium alginate containing zebularine (240 mg per 1 ml) + 50 µl of extract from 2% sodium alginate + 100 µl of DMEM HG; RA200 - 200 µl of extract from 2% sodium alginate containing retinoic acid (4.0 mg per 1 ml); RA100 - 100 µl of extract from 2% sodium alginate containing retinoic acid (4.0 mg per 1 ml) + 100 µl of DMEM HG; RA100+ALG100 - 100 µl of extract from 2% sodium alginate containing retinoic acid (4.0 mg per 1 ml) + 100 µl of extract from 2% sodium alginate; RA50+ALG50 - 50 µl of extract from 2% sodium alginate containing retinoic acid (4.0 mg per 1 ml) + 50 µl of extract from 2% sodium alginate + 100 µl of DMEM HG; ZEB100+RA100 - 100 µl of extract from 2% sodium alginate containing zebularine (240 mg per 1 ml) + 100 µl of extract from 2% sodium alginate containing retinoic acid (4.0 mg per 1 ml); ZEB50+RA50 - 50 µl of extract from 2% sodium alginate containing zebularine (240 mg per 1 ml) + 50 µl of extract from 2% sodium alginate containing retinoic acid (4.0 mg per 1 ml) + 100 µl of DMEM HG.

Journal: bioRxiv

Article Title: Development of a small-molecule epigenetic regenerative therapy. Subcutaneous administration of alginate formulations with high loads of zebularine and retinoic acid promotes tissue growth, vascularization and innervation and induces extensive epigenetic repatterning

doi: 10.1101/2024.09.18.613177

Figure Lengend Snippet: The cells were exposed to the extracts obtained by incubating alginate formulations in a DMEM HG medium. Each test was performed in 4 replicates; error bars represent the standard deviation (SD). Statistical analysis was carried out using one-way ANOVA followed by post-hoc Tukey tests; the significant differences relative to the CONTROL are marked with an asterisk *. The treatments are denoted as follows: CONTRO L - cells cultured in DMEM HG without serum; FBS - cells cultured in DMEM HG with 10% fetal bovine serum; TRITON - cells cultured in DMEM HG with 1% (v/v) Triton X100; ALG200 - 200 µl of extract from pure 2% sodium alginate formulation ALG100 - 100 µl of extract from 2% sodium alginate + 100 µl of DMEM HG; ZEB200 - 200 µl of extract from 2% sodium alginate containing zebularine (240 mg per 1 ml); ZEB100 - 100 µl of extract from 2% sodium alginate containing zebularine (240 mg per 1 ml) + 100 µl of DMEM HG; ZEB100+ALG100 - 100 µl of extract from 2% sodium alginate containing zebularine (240 mg per 1 ml) + 100 µl of extract from 2% sodium alginate; ZEB50+ALG50 - 50 µl of extract from 2% sodium alginate containing zebularine (240 mg per 1 ml) + 50 µl of extract from 2% sodium alginate + 100 µl of DMEM HG; RA200 - 200 µl of extract from 2% sodium alginate containing retinoic acid (4.0 mg per 1 ml); RA100 - 100 µl of extract from 2% sodium alginate containing retinoic acid (4.0 mg per 1 ml) + 100 µl of DMEM HG; RA100+ALG100 - 100 µl of extract from 2% sodium alginate containing retinoic acid (4.0 mg per 1 ml) + 100 µl of extract from 2% sodium alginate; RA50+ALG50 - 50 µl of extract from 2% sodium alginate containing retinoic acid (4.0 mg per 1 ml) + 50 µl of extract from 2% sodium alginate + 100 µl of DMEM HG; ZEB100+RA100 - 100 µl of extract from 2% sodium alginate containing zebularine (240 mg per 1 ml) + 100 µl of extract from 2% sodium alginate containing retinoic acid (4.0 mg per 1 ml); ZEB50+RA50 - 50 µl of extract from 2% sodium alginate containing zebularine (240 mg per 1 ml) + 50 µl of extract from 2% sodium alginate containing retinoic acid (4.0 mg per 1 ml) + 100 µl of DMEM HG.

Article Snippet: The extracts were prepared by incubating the formulations (1 ml, prepared as detailed above) in 3.3 ml DMEM HG medium (Merck, Cat. No. D6429) for 24 h at 37°C at a surface-to-volume ratio of 3 cm 2 /ml, followed by collection and centrifugation.

Techniques: Standard Deviation, Control, Cell Culture, Formulation